Figure 2: Effect of 14-week exposure to a high fat diet (HFD) or a control diet (CD) on the intra-testicular GC protein-coding transcriptome of F0 male rats.
From: High-fat diet disrupts metabolism in two generations of rats in a parent-of-origin specific manner
(a) The distribution of variance stabilising transformed (VST) expression of annotated protein-coding genes was unaffected by diet and was highly consistent across biological replicates. (b) Hierarchical clustering on the sample correlation matrix of gene expression indicated that the HFD and CD samples are highly similar. (c) Principal components analysis (PCA) was unable to distinguish samples according to diet. (d) Differential expression testing identified 3 protein-coding genes that were statistically significantly down-regulated in response to HFD (see e). A post hoc power analysis by simulation showed that the few HFD-induced changes were not due to a lack of statistical power given the same expression value and fold change range (Orange points = HFD vs. CD comparison; black points = statistically significantly differentially expressed genes between HFD and CD, including the simulated genes; purple points = simulated genes comparison). (e) The top 5 most differentially expressed genes in GCs of HFD-fed rats when compared with CD-fed rats. Mean expression indicates the expression level in GCs from CD animals, normalised for library size and averaged across 4 replicates; p-values were adjusted for multiple testing. (f) RT-qPCR validation analysis for the differentially expressed genes in (e). (Means ± SEM for n = 11–12).
