Table 1 Bacterial strains and plasmids.
Strain or Plasmid | Characteristic(s) | Source or Reference |
|---|---|---|
Brucella | ||
B. melitensis NI | Epidemic strain, smooth | This lab |
NIΔyejA1 | yejA1-deletion mutant of NI | This study |
NIΔyejA2 | yejA2-deletion mutant of NI | This study |
NIΔyejB | yejB-deletion mutant of NI | This study |
NIΔyejE | yejE-deletion mutant of NI | This study |
NIΔyejF | yejF-deletion mutant of NI | This study |
NIΔyejABEF | yej operon-deletion mutant of NI | This study |
NIΔyejEPBBRyejE | Complementation strain of NIΔyejE | This study |
E. coli | ||
DH10B | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara, leu)7697 araD139 galU galK nupG rpsL(Strr) nupG | Invitrogen |
Plasmids | ||
pEX18AP | sacB, bla, Ampr | |
pBBR1MCS | Broad-host-range plasmid, Cmr | |
pEX18ApΔyejABEF | yej operon gene deleting vector | This study |
pBBRyejE | yejE gene complementing vector | This study |
