Figure 4 | Scientific Reports

Figure 4

From: High throughput cytotoxicity screening of anti-HER2 immunotoxins conjugated with antibody fragments from phage-displayed synthetic antibody libraries

Figure 4

Binding affinities of scFvs in the presence and absence of AL1/AL2-PE38KDEL.

(A) 16 scFvs with IGKV1-NL1*01/IGHV3-23*04 germline framework were used to measure the EC50 for the scFv-HER2-ECD binding. (B) Same set of scFvs as in panel (A) were mixed with AL1-PE38KDEL with 1:1 molar ratio of the scFv:AL fragment and the EC50’s for the scFv-HER2-ECD binding were measured. (C) Same description as in panel (B) for AL2-PE38KDEL. (D) The HER2-ECD-binding EC50’s for the scFv-AL1-PE38KDEL complexes (from panel (B)) are plotted versus those for the naked scFvs (from panel (A)). The x- and y-axis show the EC50 in concentration (ng/mL) of the scFv. The EC50 values for the scFvs are listed in Supplementary Table S1. (E) Same description as in panel (D) for AL2-PE38KDEL.

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