Figure 6
Cytotoxicity screening of 92 HER2-ECD-specific scFv-AL1/AL2-PE38KDEL immunotoxins.
(A) The percentages of cell viability for N87 cells treated with scFv-AL1-PE38KDEL or scFv-AL2-PE38KDEL were determined following the procedure depicted in Fig. 1 and are shown in the x-axis and y-axis respectively. The soluble scFv in culture medium was adjusted to concentration of 0.5 nM in the presence of corresponding concentration of AL1-PE38KDEL (x-axis) or AL2-PE38KDEL (y-axis) for 1:1 molar ratio of scFv:AL fragment. Four epitope groups of these GH2 scFvs are marked in gray (M32-M62), green (M41-M61), orange (M63-M64) and purple (ungrouped). GH2 scFvs with histidine(s) in CDR-H3 are indicated by the red frame over the symbol. The detailed data for each of the scFvs are listed in Supplementary Table S2. (B) Selected GH2 scFvs were subjected to IC50 measurements for the scFv-AL1-PE38KDEL complexes. The y-axis shows the percentage of cell viability of the treated N87 cells and the x-axis shows the concentration of the scFv (1:1 molar ratio of scFv:AL fragment). The error bars were calculated with three independent repeats of the measurements. (C) Same description as in panel (B) for the scFv-AL2-PE38KDEL complexes.
