Figure 7 | Scientific Reports

Figure 7

From: High throughput cytotoxicity screening of anti-HER2 immunotoxins conjugated with antibody fragments from phage-displayed synthetic antibody libraries

Figure 7

Cytotoxicity and mean fluorescence intensity of immunotoxins on cells with different HER2 expression level.

(A,C,E,G) The IC50’s for N87 (panel (A)), SKBR3 (panel (C)), BT474 (panel (E)) and MCF7 (panel (G)) cells were measured with selected GH2 scFvs complexed with AL2-PE38KDEL in 1:1 ratio of the scFv:AL fragment. The y-axis shows percentage of cell viability (Methods); the x-axis shows the concentration of scFv. The error bars were calculated with three independent repeats of the measurements. (B,D,F,H) Mean fluorescence intensities (MFIs) for the same set of scFvs complexed with AL2-RFP (see Methods) in 1:1 ratio of the scFv:AL fragment were measured with N87 (panel (B)), SKBR3 (panel (D)), BT474 (panel (F)) and MCF7 (panel (H)) cells by flow cytometry, where the MFIs shown in the y-axis were adjusted over the background value by the cells treated with AL2-RFP without scFv. The x-axis shows the concentration of the scFv.

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