Figure 5

Th cell-associated cytokines productions in LP cells of colon after DSS-inducted colitis.
LPL from colon of DSS-treated IL-21RKO or C57BL/6 mice was isolated and cultured for 48 hours with or without anti-CD3/anti-CD28 mAbs and then cytokine production in the cultured supernatant was measured using ELISA kits. Data indicate mean ± SD of 5 mice of each group obtained from a representative of three independent experiments. Statistically significant differences are shown (**P < 0.01 or ***P < 0.001).