Figure 6

Effects of IL-21R deficiency on regulation of Treg responses in inflammatory intestine in mice.

(A) The percentage of Foxp3⁺, CD25⁺, CD25⁺Foxp3⁺ or CD25⁺Foxp3⁻ in CD4⁺ T cell in LPL of mice with DSS-induced colitis; (B) The cell numbers of CD4⁺CD25⁺, CD4⁺Foxp3⁺, CD4⁺CD25⁺Foxp3⁺ or CD4⁺CD25⁺Foxp3⁻ cells in LPL; (C) The percentage and the absolute numbers of of CD4⁺CD25⁺IL-10⁺ T cells in LPL of colon. Data indicate mean ± SD of 3 mice of either group obtained from a representative of three independent experiments. (D) LPL was cultured for 48 h under the TCR stimulations and then IL-10 production by T-LPL in the cultured supernatant was measured using ELISA kit. Data indicate mean ± SD of 5 mice of each group obtained from a representative of three independent experiments. Statistically significant differences are shown (*P < 0.05, **P < 0.01 or ***P < 0.001).