Table 1 Numerical data for individual mice used in osteoblast study, lettering corresponding to graphs in Fig. 7 and Supplementary Data S1.

From: Unique quadruple immunofluorescence assay demonstrates mitochondrial respiratory chain dysfunction in osteoblasts of aged and PolgA−/− mice

 

Mouse

NDUFB8 osteoblasts

COX-I osteoblasts

Pos

Int (+)

Int (−)

Neg

z-score

Pos

Int (+)

Int (−)

Neg

z-score

4 month wild type mice

A

100.0%

0.0%

0.0%

0.0%

−0.2645

100.0%

0.0%

0.0%

0.0%

0.1219

B

100.0%

0.0%

0.0%

0.0%

0.4862

98.0%

2.0%

0.0%

0.0%

−0.2462

C

100.0%

0.0%

0.0%

0.0%

−0.0199

100.0%

0.0%

0.0%

0.0%

0.0991

D

99.2%

0.8%

0.0%

0.0%

−0.1050

100.0%

0.0%

0.0%

0.0%

−0.0740

E

100.0%

0.0%

0.0%

0.0%

−0.1375

100.0%

0.0%

0.0%

0.0%

−0.0057

11 month wild type mice

F

61.5%

25.0%

8.7%

4.8%

−2.5793

85.6%

8.7%

2.9%

2.9%

−1.7644

G

65.1%

25.6%

8.1%

1.2%

−2.3037

46.5%

21.0%

19.8%

12.8%

−3.1797

H

90.1%

8.9%

1.0%

0.0%

−1.0015

87.1%

7.9%

3.0%

2.0%

−1.0838

I

69.3%

24.8%

3.9%

2.0%

−2.3267

82.2%

13.9%

1.0%

3.0%

−1.6834

J

69.0%

17.7%

10.6%

2.7%

−2.2288

74.3%

17.7%

7.1%

0.9%

−1.5172

K

94.3%

5.7%

0.0%

0.0%

−0.7150

72.7%

14.8%

9.1%

3.4%

−1.4310

L

77.9%

9.5%

4.2%

8.4%

−1.6644

67.4%

21.1%

9.5%

2.1%

−2.0048

M

44.4%

50.0%

5.6%

0.0%

−3.1335

75.0%

19.4%

4.6%

0.9%

−1.7746

11 month PolgA mice

N

1.9%

3.9%

8.7%

85.5%

−9.0776

12.6%

8.7%

5.8%

72.8%

−10.9406

O

3.4%

10.3%

8.0%

78.2%

−8.9053

3.4%

3.4%

2.3%

91.0%

−15.9127

P

0.0%

0.9%

4.6%

94.4%

−9.1844

3.7%

4.6%

2.8%

88.9%

−14.9438

Q

2.8%

0.9%

5.6%

90.7%

−9.7061

6.5%

4.6%

2.8%

86.1%

−14.1500

R

3.0%

12.1%

24.2%

60.6%

−6.6788

12.1%

5.1%

9.1%

73.7%

−10.0046

S

6.7%

10.6%

18.3%

64.4%

−6.6901

3.8%

5.8%

1.9%

88.5%

−13.3303

T

0.9%

1.7%

1.0%

90.3%

−8.1441

9.6%

0.9%

3.5%

86.0%

−12.7954

U

5.1%

14.7%

38.2%

42.0%

−5.7412

5.1%

5.1%

3.7%

86.0%

−12.1565

V

4.1%

0.8%

4.9%

90.2%

−9.9374

4.9%

0.8%

3.3%

91.1%

−17.1963

  1. Percentages of osteoblasts for individual mice which are positive, intermediate positive, intermediate negative and negative for NDUFB8 and COX-I protein expression are shown. Osteoblasts were classed as having normal levels (positive) if Z-scores were no more than −3SD from the mean, intermediate (+) if Z-scores were between −3SD and −4.5SD, intermediate (−) if Z-scores were between −4.5SD and −6SD, and deficient if Z-scores were more than −6SD below the mean. Average Z-scores for NDUFB8:porin and COX-I:porin are also shown.
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