Table 3 Primer sequences and product sizes of real-time PCR.
Gene name | Primer sequence (5′–3′) | Product length (bp) |
|---|---|---|
Foxp3 | F: CTGGCAAATGGTGTCTGCAAGT | 107 |
R: CTGCCCTTCTCATCCAGAAGATG | ||
RORC | F: CTGCAAGACTCATCGCCAAAG | 83 |
R: TTTCCACATGCTGGCTACACA | ||
TGF-β1 | F: GCGACTCGCCAGAGTGGTTA | 143 |
R: GTTGATGTCCACTTGCAGTGTGTTA | ||
TGF-β RI | F: GGACCCACTTCCATTTCCTTC | 125 |
R: CCATCCCACTCCTCATCCA | ||
TGF-β RII | F: GAAATTCCCAGCTTCTGGCTCA | 143 |
R: CTGTCCAGATGCTCCAGCTCAC | ||
BAMBI | F: CATACCCACATTGGAATGCTGTC | 144 |
R: TGCACCTTGGTGATAAGGTTTCTG | ||
Smad2 | F: GGCTTTACAGACCCATCAAATTCA | 161 |
R: GCACTATCACTTAGGCACTCAGCA | ||
Smad3 | F: CCAGGGCTTTGAGGCTGTCTA | 143 |
R: GCAAAGGCCCATTCAGGTG | ||
Smad4 | F: CAGCTATGCCAGAAGCCAGA | 81 |
R:GAACTCCTGGGACTTTCAACTGAC | ||
Smad6 | F: GGACAAAACAAGAAAGACGCACT | 83 |
R: AAGGAAGAGGAAGGAGGAAGAGAA | ||
Smad7 | F: CTGTCCAGATGCTGTGCCTTC | 126 |
R: TATGCCACCACGCACCAGT | ||
GAPDH | F: GCACCGTCAAGGCTGAGAAC | 138 |
R: TGGTGAAGACGCCAGTGGA |
