Figure 7 | Scientific Reports

Figure 7

From: AAV8-Mediated Angiotensin-Converting Enzyme 2 Gene Delivery Prevents Experimental Autoimmune Uveitis by Regulating MAPK, NF-κB and STAT3 Pathways

Figure 7

The expression of IFN-γ and IL-17 in response to IRBP stimulation with or without MAPK, NF-κB or STAT3 inhibitors.

Lymphocytes were extracted from the spleens and draining lymph nodes of EAU mice. The lymphocytes were pre-treated with or without the MAPK, NF-κB or STAT3 inhibitors for 30 minutes and then incubated with IRBP for 72 hours. The protein expression levels of IFN-γ and IL-17 were measured by ELISA 72 hours after stimulation by IRBP with or without the presence of the p38 MAPK inhibitor (SB203580, SB), JNK inhibitor (SP600125, SP), ERK1/2 inhibitor (PD98059, PD), NF-κB inhibitor (BAY11-7082, BAY) at a final concentration of 10 μM and STAT3 inhibitor S3I-201 at the concentration of 100 μM. The data were shown as mean ± SEM (***p < 0.001, n = 8~10). UD: undetected.

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