Figure 8 | Scientific Reports

Figure 8

From: AAV8-Mediated Angiotensin-Converting Enzyme 2 Gene Delivery Prevents Experimental Autoimmune Uveitis by Regulating MAPK, NF-κB and STAT3 Pathways

Figure 8

Involvement of the MAPK, NF-κB and STAT3 pathways in the ACE2/Ang-(1–7)/Mas axis mediated protection in EAU eye.

(a) The phosphorylation levels of p38 MAPK, ERK1/2, JNK, IκB-α and STAT3 (p-p38 MAPK, p-ERK1/2, p-JNK, p-IκB-α and p-STAT3) in the AAV8-ACE2+EAU group compared with the AAV8-eGFP+EAU and EAU groups were determined by Western blotting (*p < 0.05, **p < 0.01, ***p < 0.001, n = 4~6). The band intensities of p-p38 MAPK, p-ERK1/2 and p-JNK were normalized to p38 MAPK, ERK1/2 and JNK, respectively. The band intensities of p-IκB-α and p-STAT3 were normalized to β-actin. (b) The protein expression of p-p38 MAPK, p-ERK1/2, p-JNK, p-IκB-α and p-STAT3 in the AAV8-ACE2+EAU group compared with the AAV8-ACE2+EAU+A-779 and AAV8-ACE2 +EAU+vehicle groups was determined by Western blotting. Relative expression of p-p38 MAPK, p-ERK1/2 and p-JNK were normalized to p38 MAPK, ERK1/2 and JNK, respectively. The relative expression of p-IκB-α and p-STAT3 were normalized to β-actin. The data were shown as mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001, n = 4~6).

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