Table 1 Primer sequence for PCR.

From: Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis

Genes

Sequences (50–30)

Genebank accession

Product size

PFK

TGGATGGCGGAGATAACA

NM_001044550.1

221 bp

 

CCACTCAGAGCGGAAGGT

  

PYK

TCGCATCTTTCATCCGTAA

XM_005666189.1

124 bp

 

TCATCAAATCTCCGAACTCC

  

PPARα

TCAAGAGCCTGAGGAAACC

DQ437887.1

121 bp

 

CAAATGATAGCAGCCACAAA

  

PPARβ/δ

CAGAGCACTCGCTTCCCT

NM_214152.2

152 bp

 

GCCTGATGCCTTGTCCC

  

PPARγ

GCAGGAGCATAGCAAAGAG

DQ437884.1

58 bp

 

GGAGCGAAACTGACACCC

  

CISN

ATGAAGGTGGCAATGTAAG

NM_214276.1

228 bp

 

CCCGTCCTGAGTTGAGTG

  

ICDH

ATTCTGAAAGCCTACGACG

NM_001164007.1

143 bp

 

GAAGACTTGAGGACCTGAGC

  

OxoGDH

CGTGACCGACAGGAACATC

XM_003134891.4

239 bp

 

CGTGGACAGTGCCGTGAG

  

β-actin

TGCGGGACATCAAGGAGAAG

XM_003357928.2

216 bp

 

AGTTGAAGGTGGTCTCGTGG

  
  1. PFK, Phosphofructokinase; PYK, Pyruvate kinase; PPARα, peroxisome proliferator-activated receptor α; PPARβ/δ, peroxisome proliferator-activated receptor β/δ; PPARγ, peroxisome proliferator-activated receptor γ; CISN, citrate synthase; ICDH, isocitrate dehydrogenase; OxoGDH, oxoglutarate dehydrogenase.
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