Figure 1 | Scientific Reports

Figure 1

From: The modulation of MiR-155 and MiR-23a manipulates Klebsiella pneumoniae Adhesion on Human pulmonary Epithelial cells via Integrin α5β1 Signaling

Figure 1

MiR-155 and miR-23a expression were down-regulated in K. pneumoniae infected A549 cells and promoted K. pneumoniae adhesion.

A549 cells were exposed to increasing doses of K. pneumoniae (MOI = 0, 50, 100) for 2 hours, or fixed amount of bacteria (MOI = 100) at indicated time points (0 hr, 2 hr, 4 hr and 6 hr). The expression levels of miR-155 (A,B) and miR-23a (C,D) were examined by RT-qPCR. The Relative expression was normalized to U6 and then converted to the fold change over uninfected. A549 cells were transfected with miR-155 mimic (E), miR-23a mimic (F), miR-155 inhibitor (G) and according negative controls (miR-NC or NC) for 24 hours prior to 100 MOI of bacterial exposure. The relative K. pneumoniae adhesion at indicated time points were determined by colony counts. Relative K. p adhesion was represented after the normalization to 1 hour bacterial adhesion of miR-NC or NC. (Data are the mean ± SD and represent three individual experiments. *p < 0.05, **p < 0.01 compared with K. pneumoniae uninfected, miR-NC or NC).

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