Figure 3 | Scientific Reports

Figure 3

From: The modulation of MiR-155 and MiR-23a manipulates Klebsiella pneumoniae Adhesion on Human pulmonary Epithelial cells via Integrin α5β1 Signaling

Figure 3

MiR-155 promoted integrin/Rac1 activity during K. pneumoniae infection.

A549 cells were transfected with miR-155 mimic and according negative controls (miR-NC), or miR-155 inhibitor and according negative controls (NC) for 24 hours prior to 100 MOI of K. pneumoniae infection. (A) RT-qPCR analysis showing the mRNA levels of integrin α5 and β1. (B) Western blot analysis showing the expressions of integrin α5, integrin β1. (C) Microscopic images displaying the expressions of integrin α5 and β1 (green fluorescence, 40×). Blue fluorescence represented the nucleus staining by DAPI. (D) Fibronectin (FN)-cell Adhesion assay was performed to evaluate the effect of miR-155 mimic on uninfected A549 cells to associate with fibronectin coated plates. (E) Western blot analysis showing the pull-downed active form of Rac1 (Rac1-GTP) and total Rac1 levels. (F) Microscopic images displaying the membrane ruffles formed by polymerized F-actin (F-actin: red fluorescence, DAPI: blue fluorescence, 100×). (G) Western blot analysis showing the expressions of F-actin. (Data are the mean ± SD and represent three individual experiments. *p < 0.05, **p < 0.01 compared with miR-NC).

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