Figure 4 | Scientific Reports

Figure 4

From: The modulation of MiR-155 and MiR-23a manipulates Klebsiella pneumoniae Adhesion on Human pulmonary Epithelial cells via Integrin α5β1 Signaling

Figure 4

HMGN2 was involved in miR-155 mediated Integrin/Rac1 regulation during K. pneumoniae infection.

Western blot analysis showing the expressions of integrin α5, integrin β1 and F-actin (A) or Rac1-GTP and the total Rac1 (B) in A549 cells transfected with pEx-HMGN2 and/or siRNA-HMGN2 prior to K. pneumoniae exposure (MOI = 100 for 2 hours, same as C and D). The relative mRNA level of integrin α5 and β1 (C), the protein level of integrin α5, integrin β1 and Rac1-GTP (D) of A549 cells co-transfected with pEx-HMGN2 and miR-155 mimic prior to K. pneumoniae exposure. (E) The relative FN-cell adhesion of uninfected A549 cells transfected as (C,D). (Data are the mean ± SD and represent three individual experiments. **p < 0.01 normalized with miR-NC and pEx-NC co-transfection).

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