Figure 5 | Scientific Reports

Figure 5

From: The modulation of MiR-155 and MiR-23a manipulates Klebsiella pneumoniae Adhesion on Human pulmonary Epithelial cells via Integrin α5β1 Signaling

Figure 5

MiR-155 inhibited a known integrin transcription suppressor NFI during K. pneumoniae infection.

(A) Schematic presentation of base pairing between miR-155 and the 3′ UTR of NFIA or NFIB by erect lines. The mRNA expressions (B) and the protein levels (C) of NFIA and NFIB in A549 cells transfected with miR-155 mimic or inhibitor prior to K. pneumoniae exposure (MOI = 100 for 2 hours). (D) The schematic diagram of NFI binding motifs (TTGGC, GCCAA) on promoters of integrin α5 or β1 (−2000 bp to TSS site ATG). The primers for ChIp assay were designed for about 60–70 bp up-stream or down-stream of NFI binding motifs (−102 to +18 bp on α5 promoter, −1132 and −983 bp on β1 promoter). (E) ChIp assay showingthe NFI recruitment at the desired regions shown in (D) under the same condition as (B,C), the relative occupancy is the ratio of immunoprecipitated NFI to input DNA. (Data are the mean ± SD and represent three individual experiments. **p < 0.01 compared with miR-NC or NC).

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