Figure 5 | Scientific Reports

Figure 5

From: Yu Ping Feng San reverses cisplatin-induced multi-drug resistance in lung cancer cells via regulating drug transporters and p62/TRAF6 signalling

Figure 5

YPFS suppresses DDP-induced expression of p62/TRAF6 in A549/DDP cells.

(A) A549/DDP cells were treated with or without YPFS (1 mg/mL) for 12 hours, followed by 48 hours of DDP treatment at various concentrations. The mRNA expression of TRAF6 was determined by real-time PCR. (B) The protein expressions of p62 (~62 kDa) and TRAF6 (~58 kDa) were determined by western blotting (upper panel). Expression of α-tubulin (~55 kDa) served as a control. The full length bot of p62 is supplied in supplementary data Fig. 6. The quantitation of p62 and TRAF6 expressions were calibrated (lower panel). Values are in fold of change (X Basal) to control (no drug treatment). Each point represents the mean ± SEM, n = 3. *p < 0.05 versus DDP-treated alone.

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