Figure 6
From: Pubertal Expression of α4βδ GABAA Receptors Reduces Seizure-Like Discharges in CA1 Hippocampus
High K+ triggers less spontaneous discharge at puberty: Cell-attached recordings.
Loose seal, cell-attached recordings from CA1 hippocampal pyramidal cells (taken with the amplifier in voltage clamp mode) reveal a greater rate of spontaneous spiking in 8.5 mM K+ before puberty (Pre-pub, upper trace) compared to puberty (Pub, lower trace). (a) Representative traces. (b) Averaged data. t-test (2-tailed), t(9) = 5.7, *P = 2.8 × 10−4; n = 5–6 mice/group. Inset, current through GABAA receptor-channels is depolarizing in 8.5 mM K+ aCSF. Use of tight-seal, cell-attached recording of a CA1 hippocampal pyramidal cell (taken with the amplifier in current clamp mode) reveals a depolarization of the membrane potential upon application of 5 μM THIP (first arrow) to the slice, which repolarized to baseline upon application of 20 μM bicuculline (second arrow). This depolarization indicates outward Cl− flux. (Representative of 6 cells (1 cell/mouse)).
