Table 4 Detection of Cluster I and Cluster III nifH genes with qPCR.

From: Nitrogen fixation and nifH diversity in human gut microbiota

ID

Target copy number (copies/wet faeces g)

OTU-24

OTU-08

OTU-34

PNG

 L005

8.4 × 104

2.4 × 106

3.1 × 10 7

 L006

UD

UD

1.4 × 106

 L015

UD

6.1 × 10 7

1.4 × 108

 V001

1.1 × 105

UD

UD

 V004

5.1 × 10 6

DNQ

UD

 V009

UD

UD

UD

 V014

UD

4.0 × 106

3.9 × 10 7

 V022

UD

DNQ

1.4 × 106

 V035

UD

UD

9.6 × 107

Japanese

 J001

UD

UD

DNQ

 J002

1.6 × 10 9

UD

1.4 × 106

 J004

UD

UD

UD

 J005

UD

UD

1.8 × 105

  1. Underlining indicates OTUs detected in the cloning analysis of the corresponding samples. Each reaction was conducted in triplicate, and standards (101–107 plasmid copies) and negative controls were measured in duplicate. Quantification was performed when the target sequences were detected at ≥5 copies in at least two of the triplicate reactions. Abbreviations: UD, under detection limit; DNQ, detected but not quantified.
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