Figure 5: Minimized models applying PLS coefficient values.

To reveal the impact of autocrine and paracrine signaling, as well as material characteristics, we minimized the PLS derived model (VIP > 1.1) and systematically rebuilt it starting with the factors that had the highest VIP scores. Plots (A–D) show the predicted cell numbers calculated by the stepwise model extension with factors added with respect to decreasing VIP scores with (A) VIP > 1.6, (B,C) VIP > 1.3 and (D) VIP > 1.1.