Figure 2
EtOH-induced Golgi fission is prevented by inhibition of F-actin.
(a,b). Representative 3D SIM imaging of VA-13 cells: (a) control and treated with 35 mM EtOH for 72 h; (b) hepatocytes: from control and EtOH-fed rats. Cells were immunostained for giantin (green), NMIIA (red) and stained for F-actin by blue fluorescent phalloidin conjugate. The right panel shows high magnifications of green, red and blue channels corresponding to the perinuclear space (white boxes). (c) VA-13 cells treated with 0.2 μM Latrunculin A (LAT-A) for 24 h and EtOH treatment followed by LAT-A at 48 h. Cells were immunostained for giantin (green), NMIIA (red) and stained for F-actin by blue fluorescent phalloidin conjugate. The right panel shows high magnifications of merged, green, red and blue channels corresponding to the perinuclear space (white boxes). Merged channel is presented in color, while others in black-and-white. All confocal images were acquired with the same imaging parameters; bars, 10 μm. (d) Quantification of Pearson’s coefficient between Golgi-associated NMIIA, F-actin and giantin in cells presented in (a–c), respectively. Results are expressed as a mean ± SD from three independent experiments; *p < 0.001, **p < 0.01.
