Figure 2: GRAMD1A promotes the self-renewal of HCC stem cell and the resistance to chemotherapy. | Scientific Reports

Figure 2: GRAMD1A promotes the self-renewal of HCC stem cell and the resistance to chemotherapy.

From: GRAM domain-containing protein 1A (GRAMD1A) promotes the expansion of hepatocellular carcinoma stem cell and hepatocellular carcinoma growth through STAT5

Figure 2

(a) GSEA analysis of the correlation between GRAMD1A levels and tumor recurrence and HCC stem cells gene signature, data set was downloaded from TCGA data set. (b) Hepatosphere formation assay for the effect of GRAMD1A overexpression on self-renewal of HCC stem cells in Huh-7 and HepG2 cells. Representative micrographs (left); quantification of hepatosphere number (right). (c) SP analysis demonstrated the role of GRAMD1A in self-renewal of HCC stem cells by GRAMD1A overexpression. (d) Western blot assay of the expression of HCC stem cells markers CD130 and CD90 by overexpressing or downregulating GRAMD1A in HepG2. (e) Cell viability assay demonstrated the role of GRAMD1A in resistance to Doxorubicin by GRAMD1A overexpression. (f) TUNEL assay for the effect of GRAMD1A overexpression on resistance to Doxorubicin. Representative micrographs (left); quantification of TUNEL positive cell number (right). DAPI was used to stain nucleus. (g) Western blot assay for caspase 3 activity, PARP cleavage and the BCL-XL levels after GRAMD1A overexpression in Huh-7 and HepG2. Data are expressed as mean ± SEM. *p < 0.05.

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