Figure 2

Transient luciferase reporter gene assays with constructs containing different rs920778 allele of HOTAIR in BCPAP cells (A) or 293T cells (B). pRL-SV40 were cotransfected with these contructs to standardize transfection efficiency. Fold-changes were detected by defining the luciferase activity of cells co-transfected with pGL3-basic as 1. All experiments were performed in triplicates in three independent transfection experiments and each value represents mean ± SD. Compared with pGL3-Basic transfected cells, *P < 0.05; **P < 0.01.