Figure 1: Parasitemia, survival rate, total protein in the BALFs, and lung parasite burden in PbANKA-infected mice with or without α-lactose treatment.
(a) Survival rate. Malarial mice (n = 20) died between days 7 and 20 p.i. Malarial mice treated with α-lactose (n = 11) died between days 5 and 7 p.i. (b) Total protein levels in BALFs were measured using BCA Protein Assay. *P < 0.05; **P < 0.01 and ***P < 0.001, malarial mice on 5 days p.i., malarial mice treated with α-lactose on 5 days p.i., malarial mice on 7 days p.i., and malarial mice treated with α-lactose on 7 days p.i. vs. naive group; ξ, P < 0.05, malarial mice treated with α-lactose on 7 days p.i. vs. malarial mice at 7 days p.i.; Δ, P < 0.05, malarial mice treated with α-lactose on 7 days p.i. vs. malarial mice treated with α-lactose on 5 days p.i. There were four mice per group, and data are representative of those from two experiments. (c) Time course of parasitemia of malarial mice and malarial mice treated with α-lactose. Parasitemias are shown as mean ± SD. *P < 0.05, malarial mice treated with α-lactose on 4 days p.i. vs. malarial mice on 4 days p.i.; #P < 0.05, malarial mice treated with α-lactose on 5 days p.i. vs. malarial mice on 5 days p.i.; ξ, P < 0.05, malarial mice treated with α-lactose on 6 days p.i. vs. malarial mice on 6 days p.i. There were four mice per group, and data are representative of those from two experiments. (d) Parasite burden estimated using mRNA level of PbANKA 18S rRNA in the lungs measured by qRT-PCR. Values are means from triplicate measurements, and data are shown as −∆∆Ct values. #P < 0.05, malarial mice treated with α-lactose on 5 days p.i. vs. malarial mice on 5 days p.i. There were four mice per group, and data are representative of those from two experiments.
