Figure 4: TUNEL staining of the lung tissues of PbANKA-infected mice with or without α-lactose treatment.

Shown are representative lung sections from TUNEL staining. (a) Apoptotic lymphocytes (blue arrows) in the lungs of naive mice and mice treated with α-lactose, and apoptotic mononuclear cells (red arrows) in the lungs of malarial mice and malarial mice treated with α-lactose on 5 and 7 days p.i. Original magnification ×1,000. (b) Morphometric analysis of apoptotic cells in the lung tissues. Shown are apoptotic cells per square millimeter. Data are presented as means ± SD; experiments were performed with three mice per group. ***P < 0.001, malarial mice on 5 days p.i., malarial mice treated with α-lactose at 5 days p.i., malarial mice on 7 days p.i., and malarial mice treated with α-lactose on 7 days p.i. vs. naive group; ^##P < 0.01, malarial mice treated with α-lactose on 5 days p.i. vs. malarial mice on 5 days p.i.; Δ, P < 0.05, malarial mice treated with α-lactose on 7 days p.i. vs. malarial mice treated with α-lactose on 5 days p.i.; ξξξ, P < 0.001, malarial mice treated with α-lactose on 7 days p.i. vs. malarial mice on 7 days p.i.