Figure 1 | Scientific Reports

Figure 1

From: Osteoblasts secrete miRNA-containing extracellular vesicles that enhance expansion of human umbilical cord blood cells

Figure 1

Characterization of osteoblast-derived EVs and the RNA inside EVs.

(a) Representative transmission electron microscope image (×28,000) of EVs isolated from human osteoblasts. Scale bar: 500 nm. (b) Nanoparticle tracking analysis shows EV size distribution and concentration. (N = 3). (c,d) Representative Agilent 2100 Bioanalyzer (Pico) RNA profiles of osteoblast-EVs (c) before and (d) after RNase A treatment (100 ng/ml, 30 minutes at 37 °C). FU, fluorescent units. (N = 3). (e) Quantification of vesicular human miR-24 and miR-1 levels by qPCR in the presence or absence of exogenous synthetic miR-1 and RNase A. Data is presented as raw threshold cycle numbers (Ct values) (mean ± SD) (N = 3). n.d. denotes Ct values above 35 or not detectable.

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