Figure 2
Next-generation sequencing miRNA profiling of osteoblast-EVs.
(a) Scatter plot shows the strong correlation (R2: 0.9187) of normalized read counts (average RPKM) between cellular and vesicular miRNAs (N = 3). (b) Venn diagram shows the number of miRNAs detected in osteoblasts and EVs in comparison with ExoCarta. Numbers in brackets denote the number of highly abundant miRNAs with reads greater than 100 RPKM among all EV miRNAs. (c) Pie chart shows the normalized read count proportions of all EV-miRNAs. Only the top 15 most abundant EV-miRNAs, including let-7f-5p, let-7i-5p, let-7g-5p and let-7a-5p grouped as let7 family, are displayed. (d) Validation of highly abundant EV miRNAs by TaqMan qPCR miRNA assay. Data is presented as raw Ct values (mean ± SD) (N = 3). (e) Volcano plot (significance versus fold change) shows the significantly (P < 0.05, compared with osteoblasts by Student’s t-test) abundant (≥2-fold; red) and depleted (≤0.5-fold; green) miRNAs in EVs. Numbers in brackets denote the number of differentially expressed miRNAs. FC, fold change. (f) IPA network map shows the predicted biological functions significantly targeted by some of the highly abundant (RPKM >100) enriched EV miRNAs. Blue, EV miRNAs; Green, biological functions.
