Figure 5
EV-expanded CD34+ cells successfully engraft and re-populate NSG mice.
(a) Human chimerism level (% human CD45+ cells) (mean ± SD) in peripheral blood at several time-points after transplantation of CD34+ cells expanded in the absence (control) and presence of EVs (N = 5 mice/group). (b) The frequencies of human lymphoid and myeloid lineages in peripheral blood after 19 weeks. (c) Human chimerism level in the bone marrow after 21 weeks. CV (coefficient of variation) is 54.13% and 11.74% for control and EVs, respectively. (d) The frequencies of human CD34+ progenitors within the human CD45+ fraction in the bone marrow after 21 weeks. CV is 100.75% and 35.79% for control and EVs, respectively.
