Figure 6
Osteoblast-EVs enhance ex vivo expansion of sorted phenotypic HSCs.
(a,b) Osteoblast-EVs improve the expansion capacity of (a) total nucleated cells (TNCs) and (b) CD34+ progenitors after 10 days of expansion with SCF and Flt3L compared to cells cultured in the absence of EVs (control) (N = 4). Expansion is shown as fold change increase in total cell number compared to input. *P < 0.05, compared with control by Student’s t-test. (c) Osteoblast-EVs increase the number (mean ± SD) of phenotypic HSCs compared to control on day 10 (N = 4). **P < 0.01, compared with control by Student’s t-test. (d) EV-expanded immature UCB cells retain their differentiation capacity in CFU assay (mean ± SD) (N = 3). The frequencies of the myeloid and erythroid lineages are shown in the table. *P < 0.05, compared with control by Student’s t-test.
