Figure 7
Osteoblast-EVs stimulate the proliferation of the immature cells.
(a,b) Sorted phenotypic HSCs were loaded with CellTraceTM Violet and incubated in the absence (control) or presence of osteoblast-EVs for 5 days. Flow cytometry plots show the distribution of the progeny of (a) CD34+ progenitors and (b) CD90+ phenotypic HSCs. (c,d) The percentages (mean ± SD) of cells that have divided 1–4 times within (c) CD34+ progenitors and (d) CD90+ phenotypic HSCs (N = 2).
