Figure 2
Genetic organization of the two putative relBE loci on S. cattleya DSM46488 chromosome: relBE1sca (SCATT_20920-SCATT_20930) and relBE2sca (SCATT_39280-SCATT_39270).
(a) Scaled schematic representation of the toxin and antitoxin genes. The overlapping or separate region is shown in bold. The arrows indicate the primers used in the transcription analysis and the numbers indicate the expected size of the PCR products; (b) Transcription analysis of PCR amplification of S. cattleya relBE genes using cDNA and gene-specific primers to amplify from the 3′ end of relBs to the 5′ end of relEs (spanning both the protein-coding and the intergenic region). Lane M indicates the standard DNA size marker. Each set of the three lanes consisted of positive controls using genomic DNA as template (gDNA). PCR amplified products using cDNA prepared from log-phase S. cattleya (cDNA) and negative controls using the total RNA without reverse transcriptase (RNA). 16S rRNA was used as the positive control. The gels were cropped from the original images available at the supplementary Fig. S10.
