Figure 6
Intracellular stability of the S. cattleya antitoxin protein RelB2sca in the absence or presence of the proteinase ClpPnX (n = 1, 2, 3, 4, 5 or 6) detected by Western blotting.
The strains E. coli BL21(DE3)/pLysS with corresponding plasmids were grown in LB broth in the presence of 0.2% L-arabinose to induce the RelB2sca expression. Then 0.5 mM IPTG was added to induce the proteinases ClpPnX expression. One hour later, 200 μg/ml spectinomycin was added to inhibit protein synthesis. Samples were collected every two hours. The levels of the antitoxin protein RelB2sca were monitored by Western blotting analysis. (a) Six ClpP proteinase gene loci and one chaperone ClpX gene locus found on the linear chromosome and mega-plasmid of S. cattleya DSM46488. (b) RelB2sca protein band of Western blotting (CK denoted the control). (c) graph described the percentage of a signal for RelB2sca.
