Figure 2 | Scientific Reports

Figure 2

From: TRF2 is recruited to the pre-initiation complex as a testis-specific subunit of TFIIA/ALF to promote haploid cell gene expression

Figure 2

Trf2 is co-purified with TFIIA and ALF.

(A) Schematic of purification of testis into cytoplasmic (CE) and nuclear extract (NE) before gel filtration and tandem affinity purification. (B) Immunoblots of cytoplasmic and nuclear extracts from Trf2tag/tag and Trf2−/− testis. (C) Elution of molecular mass markers on the gel filtration column. (D) Immunoblots on fractions following gel filtration of cytoplasmic extracts from Trf2tag/tag and Trf2−/− testes. (E) Immunoblots on fractions following gel filtration performed at the indicated KCl concentrations. (F) Immunoblots on fractions following gel filtration of cytoplasmic extracts from Trf2tag/tag testes and murine embryonic stem cells. (G) Upper panel shows immunoblots on cytoplasmic extracts (Input, Inp.) from WT and Trf2+/tag heterozygous mice indicating the positions of the native and tagged proteins. The right hand lanes show the Flag immunoprecipitates from the corresponding Input fractions in the left lanes. The lower panel shows the immunoblots of the anti-ALF IP performed on the fractions eluted from the Flag IP in the upper panel.

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