Figure 3 | Scientific Reports

Figure 3

From: TRF2 is recruited to the pre-initiation complex as a testis-specific subunit of TFIIA/ALF to promote haploid cell gene expression

Figure 3

Composition of the cytoplasmic Trf2 complex.

(A) Immunoblots of the fractions obtained by tandem affinity purification. (B) SDS-PAGE and silver nitrate staining of the immunoprecipiated fractions. The locations of tagged Trf2 and the TFIIA subunits are indicated. (C) The number of peptides obtained for each protein after mass-spectrometry analysis of the precipitated fractions. (D) Immunoblots of CE, NE and chromatin pellet from non-crosslinked testis and the MNase digested chromatin from formaldehyde crosslinked chromatin. (E) Input fractions and Flag IPs from formaldehyde crosslinked chromatin. Control 1 is no antibody IP on the chromatin, control 2 is antibody and sepharose beads, but no input chromatin.

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