Figure 1: Mtb H37Ra-induced Par-4 production is associated with ER stress in macrophages.
From: The Role of Prostate Apoptosis Response-4 (Par-4) in Mycobacterium tuberculosis Infected Macrophages
(A) RAW 264.7 cells, BMDM, and THP-1 cells were infected with Mtb H37Ra at an MOI of 5, and incubated for 0–48 h. Par-4 expression was analyzed by Western blot using a Par-4 specific antibody. (B) RAW 264.7 cells were infected with at MOI of 1, 5 and 10, and incubated for indicated times. (C) ER stress sensor molecules in H37Ra (MOI = 5)-infected macrophages were detected by Western blot analysis. (D) RAW 264.7 cells were pretreated with 4-PBA (5 μM) for 1 h and then infected with H37Ra for 24 h. Tunicamycin (TM; 2 μg/mL) was used as a positive control for ER stress. Data are presented as the mean ± SD of three independent experiments.
