Figure 4: NF-κB activation is essential for Mtb H37Ra-induced Par-4 expression.
From: The Role of Prostate Apoptosis Response-4 (Par-4) in Mycobacterium tuberculosis Infected Macrophages
(A) RAW 264.7 cells were infected with Mtb H37Ra (MOI = 5), and incubated for 0–180 min. Total IκBα and p-IκBα were detected by Western blot using specific antibodies. (B) RAW 264.7 cells were pretreated with BAY 11-7085 (BAY; 1 μM) or caffeic acid phenethyl ester (CAPE; 1 μM) for 1 h and then infected with Mtb H37Ra (MOI = 5). Expression of IκBα and p-IκBα was confirmed by Western blot analysis. (C) RAW264.7 cells pretreated BAY or CAPE were infected with Mtb H37Ra for 24 h, and analyzed Par-4 expression. Data are presented as mean ± SD of three independent experiments.
