Figure 6: Par-4 regulates intracellular survival of Mtb H37Ra in macrophages.
From: The Role of Prostate Apoptosis Response-4 (Par-4) in Mycobacterium tuberculosis Infected Macrophages
The intracellular survival of Mtb H37Ra (MOI = 5) in RAW 264.7 cells was quantified by CFU analysis. (A) RAW 264.7 cells overexpressed pcDNA3.1 (1 μg/mL) and pcDNA3.1-Par-4 (1 μg/mL) and then were infected with Mtb H37Ra for 24 h. (B) RAW 264.7 cells were transfected with control siRNA (200 nM) and Par-4 siRNA (200 nM) prior to H37Ra infection for 24 h. (C) RAW 264.7 cells pretreated with NAC (20 nM) were infected with H37Ra for 24 h. Statistically significant differences are indicated by *p < 0.05, **p < 0.01, ***p < 0.001. Data are presented as mean ± SD of three independent experiments.
