Figure 7: BCG treatment induces Par-4-dependent apoptosis in PC-3 cells.
From: The Role of Prostate Apoptosis Response-4 (Par-4) in Mycobacterium tuberculosis Infected Macrophages
(A) RAW 264.7 cells or (B) PC-3 cells were infected with BCG (MOI = 5) for 3 h and then incubated for 0–48 h. Whole cell lysates were prepared and activation of Par-4, GRP78 and caspase-3 were detected using specific antibodies in Western blot analysis. (C) PC-3 cells were infected with BCG (MOI = 10) for 24 h, and localization of Par-4 and GRP78 were confirmed by immunofluorescence staining using specific antibodies against Par-4 (green), GRP78 (red) and DAPI (blue). Statistically significant differences are indicated by *p < 0.05, **p < 0.01, ***p < 0.001. Data are presented as mean ± SD of three independent experiments.
