Figure 6: Immunolocalozation of SMP30 expression in rat kidney. | Scientific Reports

Figure 6: Immunolocalozation of SMP30 expression in rat kidney.

From: Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats

Figure 6

Kidney tissues were harvested from two-month-old male rats. Tissues were formalin-fixed and paraffin-embedded. Four micrometer cross sections of the tissues were collected onto slides. Fluorescence immunohistochemical staining of kidney sections with goat anti-SMP30, mouse anti-aquaporin-1 (AQP1, renal proximal tubule marker protein), rabbit anti-Tamm-Horsfall glycoprotein (THP, the thick ascending limb of the loop of Henle marker protein), and rabbit anti-calbindin-D (renal distal tubule marker protein) antibodies were used to evaluate SMP30 localization. Nuclei counterstaining with 4,6-diamidino-2-phenylindole (DAPI) was used. Overlay was presented. (magnification 400x; scale bar = 50 μm). g, glomerulus.

Back to article page