Figure 10: Calcineurin inhibition protects podocytes against PAN-induced mitochondria dependent apoptosis. | Scientific Reports

Figure 10: Calcineurin inhibition protects podocytes against PAN-induced mitochondria dependent apoptosis.

From: Calcineurin inhibitors cyclosporin A and tacrolimus protect against podocyte injury induced by puromycin aminonucleoside in rodent models

Figure 10

(A) Flow cytometric analysis of podocyte apoptosis with or without CaN siRNA after PAN treatment (*P < 0.05). (B) Podocyte mitochondria was visualized using MitoTracker Red CMXRos. Images were taken with confocal microscope. (C) Western blot analysis of cytochrome c (cytosolic fraction) with or without CaN siRNA after PAN treatment. (D) Fluorescent staining and flow cytometric analysis (E) of the effects of CaN siRNA on PAN-induced MMP in podocytes (*P < 0.05). All experiments were performed for 3 times, and the results were shown as mean ± SD.

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