Figure 3: Exogenous IGF-1 rescues the defects in length and bone development, but not the mass of ksr2−/− mice. | Scientific Reports

Figure 3: Exogenous IGF-1 rescues the defects in length and bone development, but not the mass of ksr2−/− mice.

From: Cell non-autonomous regulation of hepatic IGF-1 and neonatal growth by Kinase Suppressor of Ras 2 (KSR2)

Figure 3

(a) Growth curves of WT and ksr2−/− injected with AdRSVeGFP (Ad-EGFP) or AdRSVhIGF-1 (Ad-IGF-1) adenovirus at PN2 (n = 16–21 per group, two-way ANOVA with repeated measures Bonferoni post hoc test was used). (b) Total IGF-1 (mouse and human) mRNA expression in PN5 ksr2+/− liver (n = 4–6 per group). For comparison, the average IGF-1 mRNA level of Ad-EGFP-injected PN5 mice was set to 1. Rps18 was used as an internal control. (c–e). Nose-to-anus length (c), bone mineral density (d), and bone mineral content (e) at 5 weeks of age of WT and ksr2−/− female mice injected with control or IGF-1-encoding adenovirus (n = 17, 7, 8, and 10 respectively). (f–h) Nose-to-anus length (f), bone mineral density (g), and bone mineral content (h) at 5 weeks of age of WT and ksr2−/− male mice injected with control or IGF-1-encoding adenovirus (n = 10, 10, 3, 3, respectively). One-way ANOVA with Bonferroni post-test with multiple comparisons was used to analyze the data in panels c-h. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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