Figure 1: Effect of SERMs on free cholesterol and nonpolar lipid distribution in THP-1 macrophages.

Cells were treated with AcLDL and vehicle (Control) or 10 μM tamoxifen (TAM), raloxifene (RAL) or toremifene (TOR). (a) Fluorescence microscopy of stainings with filipin for free cholesterol. (b) Confocal microscopy of costainings with filipin (blue) and Bodipy (green) for nonpolar lipids. Photographs are representative examples from three independent experiments.