Figure 4: Ablation of dicer in Runx2 expressing osteoblasts does not prevent glucocorticoid inhibited bone formation. | Scientific Reports

Figure 4: Ablation of dicer in Runx2 expressing osteoblasts does not prevent glucocorticoid inhibited bone formation.

From: Dicer ablation in osteoblasts by Runx2 driven cre-loxP recombination affects bone integrity, but not glucocorticoid-induced suppression of bone formation

Figure 4

(A) E15.5 femora of Dicerflox and DicerRunx2Cre mice were cultured for 3 days under osteogenic conditions with or without 10−6 M Dex. Gene expression levels of Col1a1 (A) and Osteocalcin (B) were determined by qRT-PCR. (C–E) Fluorescent micrographs of dual calcein labeling (C) and quantitative analysis of bone formation rate per bone surface (BFR/BS) (D) as well as mineral apposition rate (MAR) (E) in femoral sections of Dicerflox and DicerRunx2Cre mice under prednisolone treatment (Pred) or Control (Sham) are depicted. (F–J) Histomorphometry analysis of osteoblast number/bone perimeter (N. Ob/B.Pm) (F), osteoblast surface/bone surface (Ob.S/B.S) (G), osteocyte number/bone area (N. Ot/B.AR) (H), osteoclast number/bone perimeter (N. Oc/B.Pm) (I) and osteoclast surface/bone surface (Oc.S/B.S) (J) in femoral sections of Dicerflox and DicerRunx2Cre mice under prednisolone treatment (Pred) or Control (Sham) is shown. *p < 0.05, **p < 0.01, ***p < 0.001, n.s. not significant, OI vs OI + Dex.

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