Table 1 IC50 values for rAtPDF2.3 against yeast and plant pathogenic fungi.

From: The antifungal plant defensin AtPDF2.3 from Arabidopsis thaliana blocks potassium channels

Microorganism

IC50 (μM)

Botrytis cinerea B05.10

5.8 ± 0.0

Botrytis cinerea R16

5.8 ± 0.0

Fusarium oxysporum

4.4 ± 1.6

Fusarium culmorum

1.0 ± 0.4

Verticillium dahliae

4.4 ± 1.6

Fusarium graminearum PH-1

1.4 ± 0.0

Saccharomyces cerevisiae BY4741

8.1 ± 0.9

  1. Fungi and yeast were treated with a concentration range of rAtPDF2.3 for 48 or 24 hours, respectively, after which the IC50 values were determined microscopically for fungi and spectrophotometrically for yeast. IC50, the concentration required for 50% growth inhibition as compared to control treatment after either 24 hours for yeast, or after 48 hours for fungi. Data of at least three independent experiments are shown (n ≥ 3).