Table 2 IC50 values for rAtPDF2.3 against S. cerevisiae wild type (WT) and knockout mutants.

From: The antifungal plant defensin AtPDF2.3 from Arabidopsis thaliana blocks potassium channels

Microorganism

IC50 (μM)

P-value

Saccharomyces cerevisiae BY4741 WT

8.1 ± 0.9

NA

Saccharomyces cerevisiae BY4743 WT

3.3 ± 0.0

NA

Saccharomyces cerevisiae BY4741 ∆trk1

1.5 ± 0.0

<0.0001

Saccharomyces cerevisiae BY4741 ∆trk2

7.0 ± 0.6

0.5862

Saccharomyces cerevisiae BY4741 ∆hal5

4.0 ± 0.4

<0.0001

Saccharomyces cerevisiae BY4741 ∆prm6

7.6 ± 0.2

0.9958

Saccharomyces cerevisiae BY4741 ∆arl1

5.7 ± 0.2

0.0033

Saccharomyces cerevisiae BY4741 ∆qdr2

2.9 ± 0.2

<0.0001

Saccharomyces cerevisiae BY4741 ∆sat4

5.1 ± 0.1

0.0003

Saccharomyces cerevisiae BY4741 ∆vhc1

8.0 ± 0.2

>0.9999

Saccharomyces cerevisiae BY4741 ∆ppz2

8.6 ± 0.5

0.9868

Saccharomyces cerevisiae BY4741 ∆tok1

7.7 ± 0.5

0.9995

Saccharomyces cerevisiae BY4741 ∆nha1

9.1 ± 0.7

0.2161

Saccharomyces cerevisiae BY4741 ∆ppz1

10.1 ± 0.5

0.0120

Saccharomyces cerevisiae BY4741 ∆kch1

8.9 ± 0.3

0.8144

Saccharomyces cerevisiae BY4741 ∆kkq8

7.1 ± 0.1

0.7009

Saccharomyces cerevisiae BY4743 ∆frq1

3.1 ± 0.1

0.0935

  1. WT and knockout yeast strains were treated with a concentration range of rAtPDF2.3 for 24 hours, after which the IC50 values were determined spectrophotometrically; IC50, the concentration required for 50% growth inhibition as compared to control treatment. Mean ± SEM is shown of at least three independent experiments (n ≥ 3). ANOVA followed by Dunnett post hoc test was performed to analyse significant differences between the effect of rAtPDF2.3 on BY4741 WT yeast and knockout mutants; the adjusted P-values are presented. Unpaired Student’s t-test was performed to analyse significant differences between the effect of rAtPDF2.3 on BY4743 WT and the FRQ1 knockout mutant. NA, not applicable.
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