Table 3 IC50 values for rHsAFP1 against S. cerevisiae wild type (WT) and knockout mutants.

From: The antifungal plant defensin AtPDF2.3 from Arabidopsis thaliana blocks potassium channels

Microorganism

IC50 (μM)

P-value

Saccharomyces cerevisiae BY4741 WT

4.8 ± 0.1

NA

Saccharomyces cerevisiae BY4741 ∆trk1

2.8 ± 0.1

<0.0001

Saccharomyces cerevisiae BY4741 ∆hal5

4.6 ± 0.0

0.1073

Saccharomyces cerevisiae BY4741 ∆arl1

5.2 ± 0.1

0.0281

Saccharomyces cerevisiae BY4741 ∆qdr2

4.8 ± 0.0

0.4534

Saccharomyces cerevisiae BY4741 ∆sat4

4.7 ± 0.1

0.1865

  1. WT and knockout yeast strains were treated with a concentration range of rHsAFP1 for 24 hours, after which the IC50 values were determined spectrophotometrically; IC50, the concentration required for 50% growth inhibition as compared to control treatment. Mean ± SEM is shown of at least three independent experiments (n ≥ 3). ANOVA followed by Dunnett post hoc test was performed to analyse significant differences between the effect of rHsAFP1 on WT yeast and knockout mutants; the adjusted P-values are presented. NA, not applicable.