Figure 2
From: Dynamics of the formation of a hydrogel by a pathogenic amyloid peptide: islet amyloid polypeptide
Role of solvent molecules in IAPP fibrillisation and gelation.
(a) Rheological properties of a 4 μM IAPP solution in H2O or D2O were assessed at 25 °C with a controlled displacement of 5 × 10−3 rads and a frequency of 0.5 Hz. Dynamic moduli G’ and G” as a function of time, with the inset showing G’ and G” as a function of angular frequency ω. (b) The lag time for moduli increase, rate of increase and plateau reached are depicted. The mean of at least three independent assays is shown. Error bars represent ± SEM. (c) 4 μM IAPP was incubated in PBS with 32 μM ThT in H2O or D2O. ThT fluorescence changes were measured over time. a. u.: arbitrary units. The mean of at least three independent assays is shown. Error bars represent ± SEM. The inset shows the coefficient of variation (standard deviation over mean) as a function of time from the end of the lag phase up to beginning of plateau, i.e. to depict the variation over the elongation between the reactions in H2O and D2O. (d) The lag time, rate of increase and plateau reached are depicted. The mean of at least three independent assays is shown. Error bars represent ± SEM. **p < 0.005.
