Figure 4: All dlg mutants display smaller evoked junction currents.

(A) Representative traces of evoked synaptic current recordings of WT and dlg mutant larvae. Currents were recorded under voltage clamp with a holding voltage of −80 mV. (B) Quantification of the average current amplitude and the quantal content of WT, dlg mutants and dlg mutants over a deficiency that covers the dlg gene. All mutants show smaller currents and quantal content. (C) Representative traces of evoked synaptic current recordings of WT, GAL4-expressing larvae and larvae carrying UAS-dsRNA-dlg used as control as well as larva expressing dsRNA-dlg pre (OK6) or postsynaptically (C57), notice that only the expression of dsRNA-dlg presynaptically mimics the dlg phenotype. (D) Quantification of the average synaptic current amplitude and the average quantal content of the different genotypes in (C). (E) Representative traces of evoked synaptic current recordings of WT, GAL4 drivers in dlg mutant background alone or with UAS-DLGA or UAS-DLGS97 expressed pre or postsynaptically. (F) Quantification of the average current amplitude and the average quantal content of the genotypes shown in (E) Notice that only DLGA expressed presynaptically is able to completely rescue the dlgA mutant phenotype, while DLGS97 expressed pre and postsynaptically is able to rescue partially dlgS97 phenotype. Columns represent the average ± SEM n = 6 larvae in average 20 responses per larvae. *p < 0.05, **p < 0.01 respect to the WT. Significant respect to the mutant control.