Figure 7: Levels and localization of Cacophony channel at the synapse depend on the presence of DLGA and DLGS97.

(A) Representative confocal images of WT and dlg synaptic boutons expressing CAC1. The upper raw are images without any manipulation, note the very low signal observed in the mutants. The second row presents images that were filtered and adjusted in order to see the clusters, note that they are smaller and not always strictly associated to the periphery of the synaptic bouton. (B) Quantification of the average fluorescence signal, number of clusters per synaptic bouton and the average area of the clusters in WT and dlg mutants. (C) Synaptic currents recordings at 0.2 mM [Ca²⁺] in WT and WT and dlg mutants expressing CAC1. (D) Quantification of the synaptic current amplitudes of the genotypes showed in (C), (E) EJC average amplitudes in WT and dlg mutants expressing CAC1 at different extracellular calcium concentration from 0.2 to 2 mM. Compare with Fig. 6C. (F) Immunoprecipitation assay using anti-GFP antibody and detecting DLG with anti-DLG_PDZ. Homogenized heads of WT or ELAV-GAL4/UAS-CAC1-GFP flies were incubated with anti-GFP antibody conjugated to magnetic beads. The proteins precipitated were run in an SDS page and transfer to nitrocellulose to be blotted with anti-DLG antibody. In parallel half of the sample was run in the same gel and blotted with anti-GFP antibody. The blot with anti DLG does not show more bands than the ones shown in the figure, however the blot with GFP antibodies shows several unspecific bands. Columns represent the average ± SEM, n = 6 larvae. *p < 0.05, **p < 0.01 respect to the control.