Figure 4: linc00598 regulates cell proliferation via modulating cell cycle.
From: Long noncoding RNA linc00598 regulates CCND2 transcription and modulates the G1 checkpoint
(a) Cell proliferation was assessed through MTT assay in which linc00598 knockdown stable HEK293t cells were used. Results are expressed as means ± S.D. (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001. (b) Cell counting assays were performed using linc00598 knockdown stable HEK293t cells. The results are shown as means ± S.D. (n = 3). **P < 0.01 and ***P < 0.001. (c) Cell cycle phases of control and shlinc00598 stable HEK293t cell lines were analyzed by PI staining. Cells were fixed, stained with PI for 30 min, and analyzed by FACS. (d) Control and shlinc00598#2 stable cell lines were transfected with the indicated plasmids. Cell cycle phases of each cell line were assessed by PI staining. Cells were fixed, stained with PI for 30 min, and analyzed by FACS. (e) A model for regulation of CCND2 transcription by linc00598 through modulating accessibility of FoxO1 to the CCND2 promoter.
